Home Men Health Parkinson’s disease-like gut dysbiosis detected in early stages of the disease

Parkinson’s disease-like gut dysbiosis detected in early stages of the disease

Parkinson’s disease-like gut dysbiosis detected in early stages of the disease

In a recent article published within the journal Nature Communications, researchers performed a big cross-sectional study to profile gut microbiota dysbiosis across prodromal and early stages of Parkinson’s disease (PD), REM sleep behavior disorder (RBD), first-degree relatives of RBD (RBD-FDR), and healthy controls.

Study: Gut microbiome dysbiosis across early Parkinson’s disease, REM sleep behavior disorder and their first-degree relatives. Image Credit: Anatomy Image / Shutterstock


PD, an alpha-synucleinopathy, typically manifests because the abnormal aggregation of alpha-synuclein (α-syn) protein within the central nervous system (CNS). Dream-enactment behaviors and REM sleep without atonia characterize RBD, essentially the most specific prodromal marker of PD. Studies have confirmed that patients with video-polysomnography (v-PSG) diagnosed with RBD also suffer from constipation and α-syn pathology in ENS.

Strong scientific evidence suggests that before α-syn spreads to the CNS, α-syn pathology occurs within the enteric nervous system (ENS), which favors the gut-to-brain propagation of α-synucleinopathy. Since gut dysbiosis occurs in parallel, researchers have long hypothesized a correlation between pathological α-syn aggregation and PD-associated gut microbiota perturbations.

So, many years before motor symptoms of PD surface, constipation, the standard manifestation of a disturbed ENS on account of α-syn aggregation, could indicate the onset of PD. It justifies why understanding gut microbiota and host–microbiome interactions at prodromal stages of PD are significant.

Concerning the study

In the current study, researchers formed 4 study groups using 452 subjects, of which they retained only 441 with a high read count for further analyses. The primary group comprised 178 individuals with v-PSG-diagnosed RBD patients. The 36 early PD patients had premotor RBD features and clinically confirmed PD; furthermore, their motor symptoms had change into apparent previously five years.

The third group had 132 patients with RBD-FDR, and the fourth healthy control group comprised 130 individuals matched to people in RBD and early PD groups for age and gender. RBD patients had a greater likelihood ratio (LR) of prodromal PD than control and RBD-FDR, while controls and RBD-FDR groups had comparable levels of total LR. The team used the updated Movement Disorder Society (MDS) research criteria (2019) to compute LR and the probability of prodromal PD. A probability of prodromal PD > 80% and 30–80% were regarded as probable and possible prodromal PD, respectively.

They used the RBD questionnaire Hong Kong (RBDQ-HK) to evaluate the severity of RBD features amongst all subjects exploring its dream-associated aspects and behavioral manifestations. Likewise, the team used the Rome-IV diagnostic questionnaire to diagnose bowel disorders, e.g., functional constipation, diarrhea, and irritable bowel syndrome (IBS).

Moreover, the researchers documented stool consistency, bowel movement frequency rating (BSFS), and another gastrointestinal symptoms, e.g., dysphagia. They computed Parkinsonism using the Unified Parkinson’s Disease Rating Scale part III (UPDRS-III), wherein a complete rating >three indicated subthreshold parkinsonism.

The team collected fresh stool samples for deoxyribonucleic acid (DNA) extraction and used a NanoDrop 2000 spectrophotometer to measure its concentrations. Finally, the team used random forest modeling to discover microbial markers that effectively distinguished RBD from controls.


Investigating gut microbiota across early PD, prodromal PD, i.e., RBD, RBD-FDR, and controls revealed that in RBD patients, the general gut microbiota composition shifted to early PD, with depletion of butyrate-producing bacteria, and the overabundance of Desulfovibrio, Collinsella, and Oscillospiraceae UCG-005.

Note that Desulfovibrio is hydrogen sulfide (H2S) and lipopolysaccharide (LPS)-producing bacteria related to PD pathogenesis. The authors noted emerging PD-like microbial disturbances in younger RBD-FDR patients, e.g., increased pro-inflammatory Collinsella and decreased population of butyrate-producing Eubacterium.

The functional profile of gut microbiota dysbiosis showed an overall increase in fatty acid fermentation to lactate and ethanol. Similarly, it uncovered that in RBD-FDR, RBD, and early PD, levels of deazapurine biosynthesis reduced dramatically. Finally, the authors observed that host aspects, comparable to bowel movement frequency, gender, age, and drug use, e.g., antidepressants, osmotic laxatives, or statins, partially confounded microbial alterations in early PD, RBD, and RBD-FDR.

Quite the opposite, on the prodromal stage and early α-synucleinopathy stage, other than genera Akkermansia and Oscillospiraceae UCG-005, H2S-producing Desulfovibrio, and Collinsella  increased in RBD patients. Intriguingly, the authors noted that adopting a high-fiber weight loss plan might reverse the disruption effect of mucin-degrading bacterial genera, e.g., Akkermansia. Clearly, dietary interventions at prodromal and early α-synucleinopathy, especially amongst patients with neurological disorders, comparable to anxiety and depression, could help improve their gut condition.

On this study, 16S ribosomal ribonucleic acid (RNA) gene sequencing data suggested increased fatty acids metabolism and reduced vitamin B12 and PreQ0 biosynthesis at prodromal and early α-synucleinopathy.

The latter is a key intermediate of biosynthesis of 7-deazapurine nucleoside, which helps in synthesizing several compounds with anti-cancer effects at prodromal and early α-synucleinopathy. PreQ0 biosynthesis consistently reduced at prodromal and early α-synucleinopathy. Nevertheless, its abundance strongly co-occurs with Faecalibacterium, a butyrate-producing bacterial genus. It’s also a biomarker of PD progression.

In PD patients, the authors observed the enrichment of lactic acid bacteria (LAB). Yet, the role of lactate in PD pathogenesis stays unclear, because it conflicts with the helpful LAB effects. A possible justification is that other bacteria metabolize lactate and convert it to products which may disrupt the gut barrier.

Reduction in vitamin B12 biosynthesis or low serum B12 levels is a frequent indicator of early PD. Nevertheless, B12 deficiency appears to be related to decreased dietary intake. Since vitamin B12 is a key modulator of gut microbiota, its supplementation could help reestablish gut health.


Taken together, the study data emphasized that gut dysbiosis is already present at a much earlier stage, preceding the onset of RBD and PD, reinstating its role within the pathogenesis of α-synucleinopathy. Future prospective studies, combined with investigations of gut composition, metabolism, and inflammatory markers, would further enlighten researchers concerning the role of gut microbiota in PD-related α-syn pathologies.

Journal reference:


Please enter your comment!
Please enter your name here